Topics and Trends in Most Cited Advanced biosensing and bioanalysis techniques Papers

Ranked by citations 18 months after publication

Class of 2026 (Papers Published in 2024)

What topics and trends defined most-cited Advanced biosensing and bioanalysis techniques research in the Class of 2026?

The Class of 2026 in advanced biosensing is driven by point-of-care testing, dual-mode detection, and MXene nanomaterials. Surface plasmon resonance and refractive index sensing surged alongside nanopore sequencing, while traditional gold nanoparticle assays declined as research shifted toward multi-modal diagnostics and clinical biomarker profiling.

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Updated July 22, 2026

At a glance

Field
Advanced biosensing and bioanalysis techniques
Cohort label
Class of 2026 (2024 publications)
Papers analyzed
19,051
Papers ranked
20
Top topics in ranked papers
Point-of-care testing, electrochemical sensors, dual-mode detection, SPR sensing, MXene
Publication window
Jan 1, 2024 – Dec 31, 2024
Eligibility
Research articles; reviews excluded
Citation window
18 months post-publication
18m citation range
77–160
Data source
OpenAlex · Retrieved Jul 2026
License
CC BY 4.0

Rankings

20 papers ranked by 18-month citation count

#1 of 19,051
16018m citations

Bimetallic Single-Atom Nanozyme-Based Electrochemical-Photothermal Dual-Function Portable Immunoassay with Smartphone Imaging

Yunsen Wang et al.Analytical Chemistry202410.1021/acs.analchem.4c02606

Dianping Tang, Dietmar KnoppFuzhou University, China

Human epidermal growth factor receptor 2Biomarker detectionDual-atom nanozymeH2O2 etchingDensity functional theoryDual-mode detectionPortable devicessmartphone imagingglucose oxidase labelinghydroxyl radical generationTMB chromogenic substrateox-TMBCatalytic activityImmunoassaycolorimetric-to-signal conversionlaser irradiation photothermal readoutN-doped carbon supportPoint-of-care testing
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#3 of 19,051
11618m citations

Pt/Zn-TCPP Nanozyme-Based Flexible Immunoassay for Dual-Mode Pressure–Temperature Monitoring of Low-Abundance Proteins

Di Wu et al.Analytical Chemistry202410.1021/acs.analchem.4c01059

Dianping Tang, Xiaolong LiuFuzhou University, China

Pt/Zn-TCPP nanozymeDual-mode detectionImmunoassaypressure-temperature monitoringCarcinoembryonic antigenzinc-meso-tetrakis(4-carboxyphenyl)porphyrinphotothermal conversionflexible pressure sensor808 nm laser irradiationphotosensitizersporous materialsantigen-antibody reactionNIR camera3D-printed devicePoint-of-care testingCatalytic activitycrosstalk interferenceBiomarker detection
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#4 of 19,051
11318m citations

Peptide-Functionalized Lipid Nanoparticles for Targeted Systemic mRNA Delivery to the Brain

Emily L Han et al.Nano Letters202410.1021/acs.nanolett.4c05186

Michael J. MitchellUniversity of Pennsylvania, United States

peptide-functionalized lipid nanoparticlessystemic mRNA deliveryblood-brain barrierRVG29 peptideT7 peptideAP2 peptidemApoE peptideclick chemistrybrain endothelial cellsneuronal transfectionserum protein adsorptionintracellular traffickingendothelial transcytosisexosome secretionhepatic accumulationreceptor targetingnonviral gene delivery
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#5 of 19,051
11118m citations

Multi-pass, single-molecule nanopore reading of long protein strands

Keisuke Motone, Daphne Kontogiorgos-Heintz et al.Nature202410.1038/s41586-024-07935-7

Jeff NivalaUniversity of Washington, United States

Nanopore sequencingSingle-molecule protein sequencingClpX unfoldaseCsgG nanoporeprotein translocationtwo-residue stepping mechanismpost-translational modification mappingphosphorylation detectionmulti-pass protein rereadingbiophysical signal modelingresidue volume and chargefull-length proteoform analysisprotein domain unfoldingsingle-amino-acid substitution sequencingelectrophoretic protein threadingunfoldase motor mechanism
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#6 of 19,051
10718m citations

A self‐powered photoelectrochemical aptasensor using 3D‐carbon nitride and carbon‐based metal‐organic frameworks for high‐sensitivity detection of tetracycline in milk and water

Ying Dai et al.Journal of Food Science202410.1111/1750-3841.17398

Wenming Yang, Wanzhen XuJiangsu University, China

Self-powered biosensingGraphitic carbon nitridecarbon-doped metal-organic frameworkN-doped Cu2O@C photocathodeAntibiotic detectionFermi level differencen-type photoanodep-type photocathodePhotocurrent modulationAptamer-based sensingLimit of detectionReal sample analysisFood safety analysis
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#7 of 19,051
10218m citations

Self-designed portable dual-mode fluorescence device with custom python-based analysis software for rapid detection via dual-color FRET aptasensor with IoT capabilities

Chengquan Wang et al.Food Chemistry202410.1016/j.foodchem.2024.140190

Chengquan Wang, Jing QianJiangsu University, China

Dual-mode detectionPortable devicesPython-based analysis softwareFRET-based detectionAptamer-based sensingInternet of Things integrationRapid diagnostic platformFörster resonance energy transferPoint-of-care testingBiosensor designFluorescence detection
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#8 of 19,051
9818m citations

Ultrasensitive DNA Origami Plasmon Sensor for Accurate Detection in Circulating Tumor DNAs

Zhi Chen et al.Laser & Photonics Review202410.1002/lpor.202400035

Yonghong Shao, Han ZhangShenzhen International Institute for Biomedical Research, China

DNA origamiSurface plasmon resonance (SPR) sensingDNA scissorscirculating tumor DNAT790M mutationEGFR geneG12C mutationKRAS genenon-small cell lung cancerMutation detectionSensitivityEarly detection of lung cancerprecision diagnostics
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#9 of 19,051
9018m citations

Lamellar Ti3C2 MXene composite decorated with platinum-doped MoS2 nanosheets as electrochemical sensing functional platform for highly sensitive analysis of organophosphorus pesticides

Fei Wang et al.Food Chemistry202410.1016/j.foodchem.2024.140379

Yao Zhu, Fengxian QiuJiangsu University, China

Ti3C2 MXeneplatinum-doped MoS2lamellar compositeElectrochemical sensorPesticide detectionMXene nanocompositenanosheetsplatinum dopingMoS2 nanosheetsfunctional platformSensitivity
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#10 of 19,051
9018m citations

Tuning sensitivity of bimetallic, MXene and graphene-based SPR biosensors for rapid malaria detection: a numerical approach

Bhishma Karki et al.Journal of Computational Electronics202410.1007/s10825-024-02191-4

Bhishma KarkiTuljaram Chaturchand College Baramati, India

Surface plasmon resonance (SPR) sensingMXeneGraphene-based sensormalaria detectionbiosensor sensitivity tuningnumerical simulationbimetallic configurationsTwo-dimensional nanomaterialsOptical sensingRapid diagnostic platformgraphene surface modification
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#13 of 19,051
8518m citations

A bioswitchable delivery system for microRNA therapeutics based on a tetrahedral DNA nanostructure

Songhang Li, Taoran Tian, Tao Zhang et al.Nature Protocols202410.1038/s41596-024-01050-7

Xiaoxiao CaiSichuan University, China

bioswitchable delivery systemmicroRNA therapeuticsTetrahedral DNA nanostructureDNA nanotechnologyTargeted drug deliverystimuli-responsive nanocarriernucleic acid deliverytherapeutic RNADNA origamicontrolled releasebiocompatible nanostructuregene therapy vector
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#14 of 19,051
8418m citations

Regulating Reactive Oxygen Species over M–N–C Single-Atom Catalysts for Potential-Resolved Electrochemiluminescence

Yan Zhou et al.Journal of the American Chemical Society202410.1021/jacs.4c02986

Liuyong Hu, Wenling Gu, Chengzhou ZhuCentral China Normal University, China

potential-resolved electrochemiluminescenceM-N-C nanozymedual emitting signalsElectrochemiluminescencedissolved O2 as coreactantoxygen reduction performancehydroxyl radicalsuperoxide radicaloxophilicity modulationin situ monitoringcentral metal species modulationprincipal component analysisAntibiotic detectionactive intermediate regulationsingle luminophore system
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#16 of 19,051
8218m citations

Multifunctional methyl orange-delaminated Ti3C2 MXene for non-enzymatic/metal-free electrochemical detection of hydrogen peroxide and hydrazine

Seyyed Mehdi Khoshfetrat et al.Microchemical Journal202410.1016/j.microc.2024.111382

Seyyed Mehdi Khoshfetrat, Hossein ZhalehAyatollah Boroujerdi University, Iran

Ti3C2 MXeneEnzyme-free detectionHydrogen peroxide detectionhydrazine detectionmethyl orange delaminationMXeneElectrochemical sensormultifunctional electrode material
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#17 of 19,051
7918m citations

Plasmonic trimers designed as SERS-active chemical traps for subtyping of lung tumors

Xing Zhao, Xiaojing Liu, Dexiang Chen et al.Nature Communications202410.1038/s41467-024-50321-0

Teng Qiu, Qi HaoSoutheast University, China

Plasmonic nanostructuresSurface-enhanced Raman scattering (SERS)chemical traps4-methylbenzenethiol4-mercaptopyridine4-aminothiophenolSingle-molecule detectionbi-analyte methodlung tumor subtypingadenocarcinomasquamous carcinomaLimit of detectionField enhancementchemical affinity-directed molecular localizationfresh tissue detectionEarly detection of lung cancer
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#19 of 19,051
7718m citations

Preparation of dual recognition adsorbents based on molecularly imprinted polymers and aptamer for highly sensitive recognition and enrichment of ochratoxin A

Lingjun Geng et al.Journal of Hazardous Materials202410.1016/j.jhazmat.2024.135112

Jiashuai Sun, Yemin Guo, Xia SunShandong University of Technology, China

dual recognition adsorbentsMolecular imprintingaptamerMycotoxin detectionrecognitionenrichmentMIP-aptamer hybridDual-template imprintingSelectivityFood safety analysissynergistic recognition
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#20 of 19,051
7718m citations

Hybrid Plasmonic Biosensors with Deep Learning for Colorectal Cancer Detection

S. Kumarganesh et al.Plasmonics202410.1007/s11468-024-02568-y

Binay Kumar PandeyKnowledge Institute of Technology, India

Surface plasmon resonance (SPR) sensingdeep learningcolorectal cancer detectionPlasmonic nanostructuresconvolutional neural networksBiomarker detectionLabel-free detectionOptical sensingcancer diagnosticsmachine learning classificationField enhancementSensitivityspectral analysisPoint-of-care testing
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Dominant research themes and year-over-year shifts in Advanced biosensing and bioanalysis techniques

What Topics Define the Class of 2026?

The Class of 2026 in advanced biosensing and bioanalysis is anchored by clinical translation, optical-electrochemical synergy, and nanomaterial innovation. Point-of-care testing leads the cohort, appearing in 16.0% of top-ranked publications as researchers prioritize decentralized, rapid diagnostic platforms for immediate clinical and field applications. Electrochemical sensors (14.0%) and surface plasmon resonance (SPR) sensing (12.0%) serve as primary transduction modalities, providing ultra-sensitive, label-free quantitative monitoring of target analytes. A key hallmark of this cohort is the rapid adoption of dual-mode detection platforms (14.0%), which integrate complementary signal channels—such as electrochemical-photothermal or optical-fluorescent readouts—to cross-verify diagnostic signals and drastically reduce false positives. Furthermore, advanced 2D nanomaterials, particularly Ti3C2 MXene and MXene nanocomposites (8.0%), have emerged as essential electrode modifiers due to their superior metallic conductivity, hydrophilic surfaces, and high catalytic active sites. Coupled with electrochemiluminescence and nanopore sequencing platforms (6.0%), these bioanalysis techniques collectively emphasize portable, highly specific, and multi-analyte biosensors capable of robust performance in complex biological matrices.

Leading research themes
Leading research themes

How Did Topics Shift from the Class of 2025 to the Class of 2026?

The transition from the Class of 2025 to the Class of 2026 reveals a significant evolution from traditional single-modality assays toward multi-modal transduction and next-generation nanomaterials. Emerging optical and physical detection techniques experienced the sharpest surge: refractive index sensing and nanopore sequencing entered the top tier from near-zero baseline mentions in 2023, reflecting growing interest in single-molecule resolution and real-time physical bio-interfacing. Surface plasmon resonance (SPR) sensing doubled its presence (fold change 2.0x), while dual-mode detection rose by 75%, reflecting a field-wide movement toward orthogonal validation mechanisms. Material choices also underwent a decisive shift: Ti3C2 MXene and MXene derivatives expanded 4-fold as functional electrocatalytic scaffolds. Conversely, classic signal amplifiers saw reduced dominance; gold nanoparticles experienced a sharp contraction from 10 mentions (20.0%) down to 3 mentions (6.0%), as researchers transitioned toward enzyme-mimicking nanozymes and engineered 2D nanocomposites. Furthermore, target applications shifted away from routine food safety analysis (dropping 40%) toward high-value clinical biomarker profiling and rapid point-of-care diagnostic platforms.

How topics shifted year over year
How topics shifted year over year
Methodology

PRI identifies high-impact research using a transparent, topic-agnostic framework applied consistently across scientific domains. Bibliographic records are drawn from OpenAlex, including publication dates, citation relationships, and document types.

This ranking covers the Class of 2026 cohort: journal articles published in 2024. Reviews and other non-article document types are excluded to ensure comparability.

Research impact is quantified with an 18-month post-publication citation window—the number of citing works published within 18 months of each paper's publication date. This metric captures early impact while controlling for publication age.

An LLM-based relevance classifier then reviews each candidate's title and abstract to confirm substantive alignment with the target domain. Only papers classified as relevant appear in the final ranking.

Zheng Su, Tinsley Li, Thematic Shifts in Early-High-Impact Cancer Genomics and Diagnostics Research: A Bibliometric and Semantic Analysis. bioRxiv 2026.07.04.736459; doi: https://doi.org/10.64898/2026.07.04.736459

Full methodologyGitHubbioRxiv preprint

Cite this ranking

Pepkio Research Index (PRI). Topics and Trends in Most Cited Advanced biosensing and bioanalysis techniques Papers, Class of 2026. https://pri.pepkio.com/top-papers/advanced-biosensing-and-bioanalysis-techniques/2026. Accessed 2026-07-22.

Zheng Su, Tinsley Li, Thematic Shifts in Early-High-Impact Cancer Genomics and Diagnostics Research: A Bibliometric and Semantic Analysis. bioRxiv 2026.07.04.736459; doi: https://doi.org/10.64898/2026.07.04.736459