Strategies to overcome the challenges of low or no expression of heterologous proteins in Escherichia coli
#5PRI RANKED
Most Cited Paper
in Bacterial Genetics and Biotechnology
18 Months Post-Publication
Class of 2026
“Strategies to overcome the challenges of low or no expression of heterologous proteins in Escherichia coli”
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Topics
Escherichia coli · heterologous protein expression · codon optimization · inclusion body formation · protein solubility · chaperone co-expression · Protein purification tag · Promoter engineering · Signal peptide (Sec) · mRNA secondary structure · plasmid copy number · host strain engineering · rare codon usage · protein folding · expression vector design · temperature-induced expression · toxicity of recombinant proteins · Secretion systems · Translation efficiency · Protein purification tag
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Pepkio Research Index (PRI). Topics and Trends in Most Cited Bacterial Genetics and Biotechnology Papers, Class of 2026. https://pri.pepkio.com/top-papers/bacterial-genetics-and-biotechnology/2026. Accessed 2026-10-24. Methodology Zheng Su, Tinsley Li, Thematic Shifts in Early-High-Impact Cancer Genomics and Diagnostics Research: A Bibliometric and Semantic Analysis. bioRxiv 2026.07.04.736459; doi: https://doi.org/10.64898/2026.07.04.736459