Darwinian Evolution of Self-Replicating DNA in a Synthetic Protocell
Most Cited Paper
in Bacterial Genetics and Biotechnology
18 Months Post-Publication
Class of 2026
“Darwinian Evolution of Self-Replicating DNA in a Synthetic Protocell”
A research intelligence initiative by Pepkio
pri.pepkio.com
Topics
Synthetic protocell · Liposome compartments · PURE system · Phi29 DNA polymerase · Terminal protein · Self-replicating linear DNA template · Replication-coupled transcription-translation · Darwinian evolution in vitro · Cell-free gene expression · DNA extraction and PCR re-encapsulation · Intermittent evolution protocol · Semi-continuous evolution protocol · Next-generation sequencing of enriched mutations · Reverse engineering of mutations · Selection advantage mutations · Context-dependent mutation effects · Minimal cell-free evolving system · Synthetic cell genetic complexity · Bacteriophage-derived replication machinery · In vitro DNA self-replicator
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Pepkio Research Index (PRI). Topics and Trends in Most Cited Bacterial Genetics and Biotechnology Papers, Class of 2026. https://pri.pepkio.com/top-papers/bacterial-genetics-and-biotechnology/2026. Accessed 2026-10-24. Methodology Zheng Su, Tinsley Li, Thematic Shifts in Early-High-Impact Cancer Genomics and Diagnostics Research: A Bibliometric and Semantic Analysis. bioRxiv 2026.07.04.736459; doi: https://doi.org/10.64898/2026.07.04.736459