# Single-cell long-read sequencing-based mapping reveals specialized splicing patterns in developing and adult mouse and human brain

*PRI Rank #20 · Topics and Trends in Most Cited RNA Research and Splicing Papers, Class of 2026*

*Canonical URL: https://pri.pepkio.com/top-papers/rna-research-and-splicing/2026/rank-20*

| Field | Value |
| --- | --- |
| Rank | #20 |
| 18m citations | 61 |
| Journal | Nature Neuroscience |
| Year | 2024 |
| DOI | 10.1038/s41593-024-01616-4 |
| Corresponding authors | Hagen Tilgner |
| Institution | Weill Cornell Medicine, United States |

**Ranking page:** [Topics and Trends in Most Cited RNA Research and Splicing Papers, Class of 2026](https://pri.pepkio.com/top-papers/rna-research-and-splicing/2026)

**Paper link:** [10.1038/s41593-024-01616-4](https://doi.org/10.1038/s41593-024-01616-4)

## Topics

single-cell long-read sequencing · RNA isoforms · brain isoform map · cell-type-specific splicing · transcription start site variation · polyadenylation site variation · neurotransmitter transport genes · synapse turnover genes · postnatal day 21-to-postnatal day 28 transition · adolescent brain development · developmental isoform regulation · regional isoform regulation · mouse brain · human hippocampus · cross-species isoform conservation · human-specific isoforms · full-length isoform expression · protein architecture variation · disease-causing variants · cell subtype isoform variability

## Cite this ranking

```
Pepkio Research Index (PRI). Topics and Trends in Most Cited RNA Research and Splicing Papers, Class of 2026. https://pri.pepkio.com/top-papers/rna-research-and-splicing/2026. Accessed 2026-07-21.

Zheng Su, Tinsley Li, Thematic Shifts in Early-High-Impact Cancer Genomics and Diagnostics Research: A Bibliometric and Semantic Analysis. bioRxiv 2026.07.04.736459; doi: https://doi.org/10.64898/2026.07.04.736459
```