# Enhancing Agrobacterium-mediated plant transformation efficiency through improved ternary vector systems and auxotrophic strains

*PRI Rank #7 · Topics and Trends in Most Cited Plant tissue culture and regeneration Papers, Class of 2026*

*Canonical URL: https://pri.pepkio.com/top-papers/plant-tissue-culture-and-regeneration/2026/rank-7*

| Field | Value |
| --- | --- |
| Rank | #7 |
| 18m citations | 31 |
| Journal | Frontiers in Plant Science |
| Year | 2024 |
| DOI | 10.3389/fpls.2024.1429353 |
| Corresponding authors | Kan Wang, Keunsub Lee |
| Institution | Iowa State University, United States |

**Ranking page:** [Topics and Trends in Most Cited Plant tissue culture and regeneration Papers, Class of 2026](https://pri.pepkio.com/top-papers/plant-tissue-culture-and-regeneration/2026)

**Paper link:** [10.3389/fpls.2024.1429353](https://doi.org/10.3389/fpls.2024.1429353)

## Topics

Agrobacterium-mediated transformation · Ternary vector system · pVS1-VIR2 helper plasmid · ternary helper plasmid pKL2299A · thymidine auxotrophic Agrobacterium strains · EHA101 · EHA105 · EHA105D · LBA4404 · EHA105Thy- · LBA4404T1 · LBA4404Thy- · virA gene from pTiBo542 · vir gene operons (virG, virB, virC, virD, virE, virJ) · Transformation efficiency · GUS assay · Arabidopsis seedlings · maize B104 immature embryo transformation · Rapid transformation method · disarmed Agrobacterium strains

## Cite this ranking

```
Pepkio Research Index (PRI). Topics and Trends in Most Cited Plant tissue culture and regeneration Papers, Class of 2026. https://pri.pepkio.com/top-papers/plant-tissue-culture-and-regeneration/2026. Accessed 2026-07-15.

Zheng Su, Tinsley Li, Thematic Shifts in Early-High-Impact Cancer Genomics and Diagnostics Research: A Bibliometric and Semantic Analysis. bioRxiv 2026.07.04.736459; doi: https://doi.org/10.64898/2026.07.04.736459
```