# Systematic assessment of long-read RNA-seq methods for transcript identification and quantification

*PRI Rank #3 · Topics and Trends in Most Cited Molecular Biology Techniques and Applications Papers, Class of 2026*

*Canonical URL: https://pri.pepkio.com/top-papers/molecular-biology-techniques-and-applications/2026/rank-3*

| Field | Value |
| --- | --- |
| Rank | #3 |
| 18m citations | 124 |
| Journal | Nature Methods |
| Year | 2024 |
| DOI | 10.1038/s41592-024-02298-3 |
| Corresponding authors | Ashley M. Brooks, Christopher Vollmers, Adam Frankish, Kin Fai Au, Gloria Sheynkman, A Mortazavi, Ana Conesa, Angela N. Brooks |
| Institution | Spanish National Research Council (CSIC), Spain |

**Ranking page:** [Topics and Trends in Most Cited Molecular Biology Techniques and Applications Papers, Class of 2026](https://pri.pepkio.com/top-papers/molecular-biology-techniques-and-applications/2026)

**Paper link:** [10.1038/s41592-024-02298-3](https://doi.org/10.1038/s41592-024-02298-3)

## Topics

long-read RNA-seq · Isoform detection · transcript quantification · Transcript detection · complementary DNA sequencing · Direct RNA sequencing · Long-read RNA-Seq Genome Annotation Assessment Project · Sequencing coverage · read length · read accuracy · reference-based transcript assembly · reference-free transcript assembly · Transcript detection · Transcript detection · Multi-modal integration · transcriptome benchmarking · genome annotation · sequencing platform comparison · library preparation protocols

## Cite this ranking

```
Pepkio Research Index (PRI). Topics and Trends in Most Cited Molecular Biology Techniques and Applications Papers, Class of 2026. https://pri.pepkio.com/top-papers/molecular-biology-techniques-and-applications/2026. Accessed 2026-07-24.

Zheng Su, Tinsley Li, Thematic Shifts in Early-High-Impact Cancer Genomics and Diagnostics Research: A Bibliometric and Semantic Analysis. bioRxiv 2026.07.04.736459; doi: https://doi.org/10.64898/2026.07.04.736459
```