# Comprehensive Impurity Profiling of mRNA: Evaluating Current Technologies and Advanced Analytical Techniques

*PRI Rank #17 · Topics and Trends in Most Cited Molecular Biology Techniques and Applications Papers, Class of 2026*

*Canonical URL: https://pri.pepkio.com/top-papers/molecular-biology-techniques-and-applications/2026/rank-17*

| Field | Value |
| --- | --- |
| Rank | #17 |
| 18m citations | 31 |
| Journal | Analytical Chemistry |
| Year | 2024 |
| DOI | 10.1021/acs.analchem.3c05539 |
| Corresponding authors | Julien Camperi, Axel Guilbaud |
| Institution | Genentech, United States |

**Ranking page:** [Topics and Trends in Most Cited Molecular Biology Techniques and Applications Papers, Class of 2026](https://pri.pepkio.com/top-papers/molecular-biology-techniques-and-applications/2026)

**Paper link:** [10.1021/acs.analchem.3c05539](https://doi.org/10.1021/acs.analchem.3c05539)

## Topics

in vitro transcription (IVT) · mRNA impurities · ion-pair reversed-phase liquid chromatography · Capillary electrophoresis · Capillary electrophoresis · poly(A) tail length heterogeneity · double-stranded mRNA (dsRNA) · dsRNA 3'-loop back form · mass photometry · native mass spectrometry · mRNA aggregates · aborted poly(A) addition · partial hydrolysis · mRNA intact mass · stability-indicating methods · high-throughput impurity profiling · mRNA therapeutics quality control · mRNA sequence heterogeneity

## Cite this ranking

```
Pepkio Research Index (PRI). Topics and Trends in Most Cited Molecular Biology Techniques and Applications Papers, Class of 2026. https://pri.pepkio.com/top-papers/molecular-biology-techniques-and-applications/2026. Accessed 2026-07-24.

Zheng Su, Tinsley Li, Thematic Shifts in Early-High-Impact Cancer Genomics and Diagnostics Research: A Bibliometric and Semantic Analysis. bioRxiv 2026.07.04.736459; doi: https://doi.org/10.64898/2026.07.04.736459
```