# NCBI RefSeq: reference sequence standards through 25 years of curation and annotation

*PRI Rank #1 · Topics and Trends in Most Cited Molecular Biology Techniques and Applications Papers, Class of 2026*

*Canonical URL: https://pri.pepkio.com/top-papers/molecular-biology-techniques-and-applications/2026/rank-1*

| Field | Value |
| --- | --- |
| Rank | #1 |
| 18m citations | 239 |
| Journal | Nucleic Acids Research |
| Year | 2024 |
| DOI | 10.1093/nar/gkae1038 |
| Corresponding authors | Tamara Goldfarb |
| Institution | National Institutes of Health, United States |

**Ranking page:** [Topics and Trends in Most Cited Molecular Biology Techniques and Applications Papers, Class of 2026](https://pri.pepkio.com/top-papers/molecular-biology-techniques-and-applications/2026)

**Paper link:** [10.1093/nar/gkae1038](https://doi.org/10.1093/nar/gkae1038)

## Topics

NCBI RefSeq · reference sequences · genome annotation · transcript annotation · protein annotation · eukaryotic annotation · prokaryotic RefSeq · viral RefSeq · Transcriptomic data · Ortholog mapping · comparative genomics · expert curation · automatic annotation processes · sequencing technologies · taxonomic representation · gene function interpretation · sequence variation · transcriptomics · proteomics · Quality control

## Cite this ranking

```
Pepkio Research Index (PRI). Topics and Trends in Most Cited Molecular Biology Techniques and Applications Papers, Class of 2026. https://pri.pepkio.com/top-papers/molecular-biology-techniques-and-applications/2026. Accessed 2026-07-24.

Zheng Su, Tinsley Li, Thematic Shifts in Early-High-Impact Cancer Genomics and Diagnostics Research: A Bibliometric and Semantic Analysis. bioRxiv 2026.07.04.736459; doi: https://doi.org/10.64898/2026.07.04.736459
```