# Ultrafast bisulfite sequencing detection of 5-methylcytosine in DNA and RNA

*PRI Rank #19 · Topics and Trends in Most Cited Epigenetics and DNA Methylation Papers, Class of 2026*

*Canonical URL: https://pri.pepkio.com/top-papers/epigenetics-and-dna-methylation/2026/rank-19*

| Field | Value |
| --- | --- |
| Rank | #19 |
| 18m citations | 70 |
| Journal | Nature Biotechnology |
| Year | 2024 |
| DOI | 10.1038/s41587-023-02034-w |
| Corresponding authors | Qing Dai, Chuan He |
| Institution | The University of Chicago, United States |

**Ranking page:** [Topics and Trends in Most Cited Epigenetics and DNA Methylation Papers, Class of 2026](https://pri.pepkio.com/top-papers/epigenetics-and-dna-methylation/2026)

**Paper link:** [10.1038/s41587-023-02034-w](https://doi.org/10.1038/s41587-023-02034-w)

## Topics

ultrafast bisulfite sequencing · UBS-seq · 5-methylcytosine · 5mC · m5C · m5C · C-to-U conversion · cfDNA · mouse embryonic stem cells · m5C stoichiometry · highly structured RNA sequences · NSUN2 · HeLa mRNA · 5'-regions of mammalian mRNA · mRNA translation regulation · DNA damage reduction · low-input library construction

## Cite this ranking

```
Pepkio Research Index (PRI). Topics and Trends in Most Cited Epigenetics and DNA Methylation Papers, Class of 2026. https://pri.pepkio.com/top-papers/epigenetics-and-dna-methylation/2026. Accessed 2026-07-21.

Zheng Su, Tinsley Li, Thematic Shifts in Early-High-Impact Cancer Genomics and Diagnostics Research: A Bibliometric and Semantic Analysis. bioRxiv 2026.07.04.736459; doi: https://doi.org/10.64898/2026.07.04.736459
```