# Engineering thermostability of industrial enzymes for enhanced application performance

*PRI Rank #2 · Topics and Trends in Most Cited Enzyme Production and Characterization Papers, Class of 2026*

*Canonical URL: https://pri.pepkio.com/top-papers/enzyme-production-and-characterization/2026/rank-2*

| Field | Value |
| --- | --- |
| Rank | #2 |
| 18m citations | 65 |
| Journal | International Journal of Biological Macromolecules |
| Year | 2024 |
| DOI | 10.1016/j.ijbiomac.2024.139067 |
| Corresponding authors | Jingwen Zhou, Xinglong Wang |
| Institution | Jiangnan University, China |

**Ranking page:** [Topics and Trends in Most Cited Enzyme Production and Characterization Papers, Class of 2026](https://pri.pepkio.com/top-papers/enzyme-production-and-characterization/2026)

**Paper link:** [10.1016/j.ijbiomac.2024.139067](https://doi.org/10.1016/j.ijbiomac.2024.139067)

## Topics

thermostability engineering · Industrial enzymes · Thermostability · Rational design · directed evolution · Enzyme engineering · thermal stability · Rational design · disulfide bond engineering · consensus sequence approach · Computer-aided protein design · site-directed mutagenesis · Thermophilic enzymes · enzyme activity at high temperature · industrial biocatalysis

## Cite this ranking

```
Pepkio Research Index (PRI). Topics and Trends in Most Cited Enzyme Production and Characterization Papers, Class of 2026. https://pri.pepkio.com/top-papers/enzyme-production-and-characterization/2026. Accessed 2026-07-24.

Zheng Su, Tinsley Li, Thematic Shifts in Early-High-Impact Cancer Genomics and Diagnostics Research: A Bibliometric and Semantic Analysis. bioRxiv 2026.07.04.736459; doi: https://doi.org/10.64898/2026.07.04.736459
```