# De novo 2′-fucosyllactose biosynthesis using glucose as the sole carbon source by multiple engineered Bacillus subtilis

*PRI Rank #11 · Topics and Trends in Most Cited Enzyme Production and Characterization Papers, Class of 2026*

*Canonical URL: https://pri.pepkio.com/top-papers/enzyme-production-and-characterization/2026/rank-11*

| Field | Value |
| --- | --- |
| Rank | #11 |
| 18m citations | 25 |
| Journal | Metabolic Engineering |
| Year | 2024 |
| DOI | 10.1016/j.ymben.2024.12.004 |
| Corresponding authors | Yanfeng Liu, Long Liu |
| Institution | Jiangnan University, China |

**Ranking page:** [Topics and Trends in Most Cited Enzyme Production and Characterization Papers, Class of 2026](https://pri.pepkio.com/top-papers/enzyme-production-and-characterization/2026)

**Paper link:** [10.1016/j.ymben.2024.12.004](https://doi.org/10.1016/j.ymben.2024.12.004)

## Topics

2'-fucosyllactose · de novo biosynthesis · Bacillus subtilis · glucose as sole carbon source · metabolic engineering · fucosyltransferase · GDP-L-fucose pathway · lactose biosynthesis · human milk oligosaccharide · fucosylation · salvage pathway · heterologous expression · cofactor regeneration · fed-batch fermentation

## Cite this ranking

```
Pepkio Research Index (PRI). Topics and Trends in Most Cited Enzyme Production and Characterization Papers, Class of 2026. https://pri.pepkio.com/top-papers/enzyme-production-and-characterization/2026. Accessed 2026-07-24.

Zheng Su, Tinsley Li, Thematic Shifts in Early-High-Impact Cancer Genomics and Diagnostics Research: A Bibliometric and Semantic Analysis. bioRxiv 2026.07.04.736459; doi: https://doi.org/10.64898/2026.07.04.736459
```