# Split crRNA with CRISPR-Cas12a enabling highly sensitive and multiplexed detection of RNA and DNA

*PRI Rank #4 · Topics and Trends in Most Cited CRISPR and Genetic Engineering Papers, Class of 2026*

*Canonical URL: https://pri.pepkio.com/top-papers/crispr-and-genetic-engineering/2026/rank-4*

| Field | Value |
| --- | --- |
| Rank | #4 |
| 18m citations | 134 |
| Journal | Nature Communications |
| Year | 2024 |
| DOI | 10.1038/s41467-024-52691-x |
| Corresponding authors | Jie Qiao, Yi Liu |
| Institution | Wuhan Polytechnic University, China |

**Ranking page:** [Topics and Trends in Most Cited CRISPR and Genetic Engineering Papers, Class of 2026](https://pri.pepkio.com/top-papers/crispr-and-genetic-engineering/2026)

**Paper link:** [10.1038/s41467-024-52691-x](https://doi.org/10.1038/s41467-024-52691-x)

## Topics

crRNA · Cas12a · SCas12a system · Amplification-free detection · Nucleic acid detection · microRNA detection · mature miRNA versus pre-miRNA differentiation · Point mutation detection · miR-21 · cervical cancer plasma samples · HPV detection · RPA combination · Attomolar detection · lateral flow assay · fluorescence-based detection · Multiplexed detection · Trans-cleavage activity · long RNA detection · biomarker quantification

## Cite this ranking

```
Pepkio Research Index (PRI). Topics and Trends in Most Cited CRISPR and Genetic Engineering Papers, Class of 2026. https://pri.pepkio.com/top-papers/crispr-and-genetic-engineering/2026. Accessed 2026-07-21.

Zheng Su, Tinsley Li, Thematic Shifts in Early-High-Impact Cancer Genomics and Diagnostics Research: A Bibliometric and Semantic Analysis. bioRxiv 2026.07.04.736459; doi: https://doi.org/10.64898/2026.07.04.736459
```