# Machine Learning-Assisted, Dual-Channel CRISPR/Cas12a Biosensor-In-Microdroplet for Amplification-Free Nucleic Acid Detection for Food Authenticity Testing

*PRI Rank #19 · Topics and Trends in Most Cited CRISPR and Genetic Engineering Papers, Class of 2026*

*Canonical URL: https://pri.pepkio.com/top-papers/crispr-and-genetic-engineering/2026/rank-19*

| Field | Value |
| --- | --- |
| Rank | #19 |
| 18m citations | 72 |
| Journal | ACS Nano |
| Year | 2024 |
| DOI | 10.1021/acsnano.4c10823 |
| Corresponding authors | Shengying Ye, Shuli Man, Long Ma |
| Institution | Tianjin University of Science & Technology, China |

**Ranking page:** [Topics and Trends in Most Cited CRISPR and Genetic Engineering Papers, Class of 2026](https://pri.pepkio.com/top-papers/crispr-and-genetic-engineering/2026)

**Paper link:** [10.1021/acsnano.4c10823](https://doi.org/10.1021/acsnano.4c10823)

## Topics

CRISPR/Cas12a biosensor · microdroplet platform · CC-drop · Trans-cleavage activity · crRNA · single-stranded DNA reporter · picoliter reaction volume · Amplification-free detection · random forest machine learning model · smartphone App · fluorescence lighting-up detection · ND1 gene · IL-2 gene · meat species authenticity · food authenticity testing · truncated DNA detection · deep processed meat products · Multiplexed detection · digital single-molecule detection

## Cite this ranking

```
Pepkio Research Index (PRI). Topics and Trends in Most Cited CRISPR and Genetic Engineering Papers, Class of 2026. https://pri.pepkio.com/top-papers/crispr-and-genetic-engineering/2026. Accessed 2026-07-21.

Zheng Su, Tinsley Li, Thematic Shifts in Early-High-Impact Cancer Genomics and Diagnostics Research: A Bibliometric and Semantic Analysis. bioRxiv 2026.07.04.736459; doi: https://doi.org/10.64898/2026.07.04.736459
```