# Efficient site-specific integration of large genes in mammalian cells via continuously evolved recombinases and prime editing

*PRI Rank #13 · Topics and Trends in Most Cited CRISPR and Genetic Engineering Papers, Class of 2026*

*Canonical URL: https://pri.pepkio.com/top-papers/crispr-and-genetic-engineering/2026/rank-13*

| Field | Value |
| --- | --- |
| Rank | #13 |
| 18m citations | 101 |
| Journal | Nature Biomedical Engineering |
| Year | 2024 |
| DOI | 10.1038/s41551-024-01227-1 |
| Corresponding authors | David R. Liu |
| Institution | Broad Institute of MIT and Harvard, United States |

**Ranking page:** [Topics and Trends in Most Cited CRISPR and Genetic Engineering Papers, Class of 2026](https://pri.pepkio.com/top-papers/crispr-and-genetic-engineering/2026)

**Paper link:** [10.1038/s41551-024-01227-1](https://doi.org/10.1038/s41551-024-01227-1)

## Topics

PASSIGE · Prime editing · Programmable recombination · Phage-assisted continuous evolution · Programmable recombination · evoBxb1 · eeBxb1 · Targeted DNA insertion · Programmable recombination · safe-harbour loci · primary human fibroblasts · PASTE · Targeted DNA insertion · Targeted DNA insertion · Targeted DNA insertion · therapeutically relevant genomic sites · donor integration efficiency · mammalian genome engineering

## Cite this ranking

```
Pepkio Research Index (PRI). Topics and Trends in Most Cited CRISPR and Genetic Engineering Papers, Class of 2026. https://pri.pepkio.com/top-papers/crispr-and-genetic-engineering/2026. Accessed 2026-07-21.

Zheng Su, Tinsley Li, Thematic Shifts in Early-High-Impact Cancer Genomics and Diagnostics Research: A Bibliometric and Semantic Analysis. bioRxiv 2026.07.04.736459; doi: https://doi.org/10.64898/2026.07.04.736459
```