# Reconstruction, simulation and analysis of enzyme-constrained metabolic models using GECKO Toolbox 3.0

*PRI Rank #18 · Topics and Trends in Most Cited Biofuel production and bioconversion Papers, Class of 2026*

*Canonical URL: https://pri.pepkio.com/top-papers/biofuel-production-and-bioconversion/2026/rank-18*

| Field | Value |
| --- | --- |
| Rank | #18 |
| 18m citations | 49 |
| Journal | Nature Protocols |
| Year | 2024 |
| DOI | 10.1038/s41596-023-00931-7 |
| Corresponding authors | Eduard J. Kerkhoven |
| Institution | Chalmers University of Technology, Sweden |

**Ranking page:** [Topics and Trends in Most Cited Biofuel production and bioconversion Papers, Class of 2026](https://pri.pepkio.com/top-papers/biofuel-production-and-bioconversion/2026)

**Paper link:** [10.1038/s41596-023-00931-7](https://doi.org/10.1038/s41596-023-00931-7)

## Topics

GECKO Toolbox 3.0 · Enzyme-constrained metabolic models · Enzyme-constrained metabolic models · genome-scale metabolic models · Enzyme kinetics · Enzyme turnover number (kcat) · Proteome constraints · flux balance analysis · metabolic reconstruction · enzyme usage · Proteome constraints · Enzyme turnover number (kcat) · metabolic simulation · systems biology · constraint-based modeling

## Cite this ranking

```
Pepkio Research Index (PRI). Topics and Trends in Most Cited Biofuel production and bioconversion Papers, Class of 2026. https://pri.pepkio.com/top-papers/biofuel-production-and-bioconversion/2026. Accessed 2026-07-21.

Zheng Su, Tinsley Li, Thematic Shifts in Early-High-Impact Cancer Genomics and Diagnostics Research: A Bibliometric and Semantic Analysis. bioRxiv 2026.07.04.736459; doi: https://doi.org/10.64898/2026.07.04.736459
```