# Establishing a synthetic orthogonal replication system enables accelerated evolution in <i>E. coli</i>

*PRI Rank #6 · Topics and Trends in Most Cited Bacterial Genetics and Biotechnology Papers, Class of 2026*

*Canonical URL: https://pri.pepkio.com/top-papers/bacterial-genetics-and-biotechnology/2026/rank-6*

| Field | Value |
| --- | --- |
| Rank | #6 |
| 18m citations | 54 |
| Journal | Science |
| Year | 2024 |
| DOI | 10.1126/science.adk1281 |
| Corresponding authors | Rongzhen Tian, Jason W. Chin |
| Institution | Medical Research Council Laboratory of Molecular Biology, United Kingdom |

**Ranking page:** [Topics and Trends in Most Cited Bacterial Genetics and Biotechnology Papers, Class of 2026](https://pri.pepkio.com/top-papers/bacterial-genetics-and-biotechnology/2026)

**Paper link:** [10.1126/science.adk1281](https://doi.org/10.1126/science.adk1281)

## Topics

Orthogonal replication · Orthogonal replication · Orthogonal replication · Continuous evolution · mutagenesis rate elevation · tigecycline resistance evolution · GFP fluorescence evolution · Escherichia coli · mutant O-DNAPs · host polymerase orthogonality · replicon cargo capacity · mutation rate engineering · Directed evolution · synthetic biology · selective replication

## Cite this ranking

```
Pepkio Research Index (PRI). Topics and Trends in Most Cited Bacterial Genetics and Biotechnology Papers, Class of 2026. https://pri.pepkio.com/top-papers/bacterial-genetics-and-biotechnology/2026. Accessed 2026-10-28.

Zheng Su, Tinsley Li, Thematic Shifts in Early-High-Impact Cancer Genomics and Diagnostics Research: A Bibliometric and Semantic Analysis. bioRxiv 2026.07.04.736459; doi: https://doi.org/10.64898/2026.07.04.736459
```