# Strategies to overcome the challenges of low or no expression of heterologous proteins in Escherichia coli

*PRI Rank #5 · Topics and Trends in Most Cited Bacterial Genetics and Biotechnology Papers, Class of 2026*

*Canonical URL: https://pri.pepkio.com/top-papers/bacterial-genetics-and-biotechnology/2026/rank-5*

| Field | Value |
| --- | --- |
| Rank | #5 |
| 18m citations | 55 |
| Journal | Biotechnology Advances |
| Year | 2024 |
| DOI | 10.1016/j.biotechadv.2024.108417 |
| Corresponding authors | Huimin Yu |
| Institution | Tsinghua University, China |

**Ranking page:** [Topics and Trends in Most Cited Bacterial Genetics and Biotechnology Papers, Class of 2026](https://pri.pepkio.com/top-papers/bacterial-genetics-and-biotechnology/2026)

**Paper link:** [10.1016/j.biotechadv.2024.108417](https://doi.org/10.1016/j.biotechadv.2024.108417)

## Topics

Escherichia coli · heterologous protein expression · codon optimization · inclusion body formation · protein solubility · chaperone co-expression · Protein purification tag · Promoter engineering · Signal peptide (Sec) · mRNA secondary structure · plasmid copy number · host strain engineering · rare codon usage · protein folding · expression vector design · temperature-induced expression · toxicity of recombinant proteins · Secretion systems · Translation efficiency · Protein purification tag

## Cite this ranking

```
Pepkio Research Index (PRI). Topics and Trends in Most Cited Bacterial Genetics and Biotechnology Papers, Class of 2026. https://pri.pepkio.com/top-papers/bacterial-genetics-and-biotechnology/2026. Accessed 2026-10-28.

Zheng Su, Tinsley Li, Thematic Shifts in Early-High-Impact Cancer Genomics and Diagnostics Research: A Bibliometric and Semantic Analysis. bioRxiv 2026.07.04.736459; doi: https://doi.org/10.64898/2026.07.04.736459
```